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s mutans ua159 strain (ATCC)

Name: s mutans ua159 strain
Brand: ATCC
Rating: 94 (233 reviews)

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ATCC s mutans ua159 strain

The ∆ gtfB mutant lacking GtfB exhibited increased resistance to ClyR. (A) Coomassie-stained SDS-PAGE gel showing the Gtfs content in the culture supernatant of S. mutans <t>UA159</t> and the ∆ gtfB mutant, with arrows indicating GtfC and GtfD bands. (B) Zymogram assay detecting water-insoluble EPS synthesis activity of Gtfs. Bands corresponding to water-insoluble EPS are labeled. (C) 10-fold serial dilutions of bacteria were spotted onto BHIA plates 1 h and 7 h after treatment with and without ClyR, followed by observation and CFU counting after 48 h. (D) Survival rates of S. mutans UA159 and ∆ gtfB were assessed 1 h and 7 h after exposure to ClyR (* p < 0.05, ** p < 0.01).

S Mutans Ua159 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 233 article reviews

s mutans ua159 strain - by Bioz Stars, 2026-02

94/100 stars

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1) Product Images from "Water-insoluble exopolysaccharide synthesized by glucosyltransferases mediates the antibacterial activity of ClyR against Streptococcus mutans"

Article Title: Water-insoluble exopolysaccharide synthesized by glucosyltransferases mediates the antibacterial activity of ClyR against Streptococcus mutans

Journal: Journal of Oral Microbiology

doi: 10.1080/20002297.2025.2566894

Figure Legend Snippet: The ∆ gtfB mutant lacking GtfB exhibited increased resistance to ClyR. (A) Coomassie-stained SDS-PAGE gel showing the Gtfs content in the culture supernatant of S. mutans UA159 and the ∆ gtfB mutant, with arrows indicating GtfC and GtfD bands. (B) Zymogram assay detecting water-insoluble EPS synthesis activity of Gtfs. Bands corresponding to water-insoluble EPS are labeled. (C) 10-fold serial dilutions of bacteria were spotted onto BHIA plates 1 h and 7 h after treatment with and without ClyR, followed by observation and CFU counting after 48 h. (D) Survival rates of S. mutans UA159 and ∆ gtfB were assessed 1 h and 7 h after exposure to ClyR (* p < 0.05, ** p < 0.01).

Techniques Used: Mutagenesis, Staining, SDS Page, Activity Assay, Labeling, Bacteria

Biomass and structure of S. mutans UA159 and ∆gtfB biofilms after ClyR treatment. (A) Biofilm morphology and biomass of S. mutans UA159 and ∆ gtfB were examined using SEM (× 20,000 magnification) and CLSM. (B) Quantification of biofilm thickness loss, water-insoluble EPS loss, and bacterial reduction after ClyR treatment for both strains (** p < 0.01).

Figure Legend Snippet: Biomass and structure of S. mutans UA159 and ∆gtfB biofilms after ClyR treatment. (A) Biofilm morphology and biomass of S. mutans UA159 and ∆ gtfB were examined using SEM (× 20,000 magnification) and CLSM. (B) Quantification of biofilm thickness loss, water-insoluble EPS loss, and bacterial reduction after ClyR treatment for both strains (** p < 0.01).

Techniques Used:

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Journal: Journal of Oral Microbiology

Article Title: Water-insoluble exopolysaccharide synthesized by glucosyltransferases mediates the antibacterial activity of ClyR against Streptococcus mutans

doi: 10.1080/20002297.2025.2566894

Figure Lengend Snippet: The ∆ gtfB mutant lacking GtfB exhibited increased resistance to ClyR. (A) Coomassie-stained SDS-PAGE gel showing the Gtfs content in the culture supernatant of S. mutans UA159 and the ∆ gtfB mutant, with arrows indicating GtfC and GtfD bands. (B) Zymogram assay detecting water-insoluble EPS synthesis activity of Gtfs. Bands corresponding to water-insoluble EPS are labeled. (C) 10-fold serial dilutions of bacteria were spotted onto BHIA plates 1 h and 7 h after treatment with and without ClyR, followed by observation and CFU counting after 48 h. (D) Survival rates of S. mutans UA159 and ∆ gtfB were assessed 1 h and 7 h after exposure to ClyR (* p < 0.05, ** p < 0.01).

Article Snippet: The S. mutans UA159 strain was obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA), and its derivative was provided by the State Key Laboratory of Oral Diseases at Sichuan University.

Techniques: Mutagenesis, Staining, SDS Page, Activity Assay, Labeling, Bacteria

Journal: Journal of Oral Microbiology

Article Title: Water-insoluble exopolysaccharide synthesized by glucosyltransferases mediates the antibacterial activity of ClyR against Streptococcus mutans

doi: 10.1080/20002297.2025.2566894

Figure Lengend Snippet: Biomass and structure of S. mutans UA159 and ∆gtfB biofilms after ClyR treatment. (A) Biofilm morphology and biomass of S. mutans UA159 and ∆ gtfB were examined using SEM (× 20,000 magnification) and CLSM. (B) Quantification of biofilm thickness loss, water-insoluble EPS loss, and bacterial reduction after ClyR treatment for both strains (** p < 0.01).

Techniques:

Inhibitory effect of BBR on the growth of bacteria. ( A ) The MIC and MBC values of BBR against planktonic bacteria. 10 6 CFU/mL bacteria in BHI broth were incubated with final concentrations of BBR ranging from 25 to 400 μg/mL anaerobically at 37°C for 24 h. The MIC was defined as the lowest concentration of BBR that inhibited visible bacterial growth. At the termination of the MIC assay, a volume of the culture was struck on BHIA and incubated to observe growth. The MBC was defined as the lowest concentration that yielded no colony growth by subculturing on BHIA plates. ( B–F ) The 24 h growth curve of planktonic S. mutans UA159, S. mutans ATCC 25175, S. mutans GS-5, S. gordonii DL-1, and S. mitis ATCC 6249 incubated under anaerobic conditions with/without treatment of BBR in a 96-well plate. The absorbance of each well was recorded every hour. ( G and H ) The short-term antibacterial effect of BBR on S. mutans UA159 was assessed by measuring the average number of CFU. S. mutans UA159 was treated with BBR for 5 min ( G ) and 10 min ( H ). The suspensions were then diluted and plated onto BHIA plates and incubated under anaerobic conditions at 37°C for 24 h to determine CFU counts. Values represent the means ± SD from three independent experiments (** P ＜0.01, *** P ＜0.001).

Journal: Microbiology Spectrum

Article Title: Inhibitory effects of berberine against Streptococcus mutans : an in vitro insight on its anticaries potential

doi: 10.1128/spectrum.00700-25

Figure Lengend Snippet: Inhibitory effect of BBR on the growth of bacteria. ( A ) The MIC and MBC values of BBR against planktonic bacteria. 10 6 CFU/mL bacteria in BHI broth were incubated with final concentrations of BBR ranging from 25 to 400 μg/mL anaerobically at 37°C for 24 h. The MIC was defined as the lowest concentration of BBR that inhibited visible bacterial growth. At the termination of the MIC assay, a volume of the culture was struck on BHIA and incubated to observe growth. The MBC was defined as the lowest concentration that yielded no colony growth by subculturing on BHIA plates. ( B–F ) The 24 h growth curve of planktonic S. mutans UA159, S. mutans ATCC 25175, S. mutans GS-5, S. gordonii DL-1, and S. mitis ATCC 6249 incubated under anaerobic conditions with/without treatment of BBR in a 96-well plate. The absorbance of each well was recorded every hour. ( G and H ) The short-term antibacterial effect of BBR on S. mutans UA159 was assessed by measuring the average number of CFU. S. mutans UA159 was treated with BBR for 5 min ( G ) and 10 min ( H ). The suspensions were then diluted and plated onto BHIA plates and incubated under anaerobic conditions at 37°C for 24 h to determine CFU counts. Values represent the means ± SD from three independent experiments (** P ＜0.01, *** P ＜0.001).

Article Snippet: The bacterial strain S. mutans UA159 was obtained from the American Type Culture Collection (ATCC).

Techniques: Bacteria, Incubation, Concentration Assay, Subculturing Assay

Effect of BBR on the biofilm formation of S. mutans . In these assays, BBR was incubated with S. mutans in BHIS for 24 h to evaluate its effect on the biofilm formation. ( A ) The crystal violet staining of S. mutans 24 h biofilms under the treatment of BBR. The images were taken from a 24-well cell culture plate. ( B ) The results of the crystal violet staining assay applied to S. mutans biofilms after treated with different concentrations of BBR for 24 h in BHIS. ( C ) SEM micro-images of S. mutans 24 h biofilms on glass coverslips. In the control group, S. mutans bacteria formed dense biofilms covered with large amounts of EPS (red arrows). Disrupted biofilm skeleton (yellow arrows) and enlarged biofilm pores (green arrows) were observed under the treatment of 50 µg/mL of BBR. Only a small number of morphologically irregular S. mutans and cell contents were observed under the treatment of 100 µg/mL and 200 µg/mL of BBR (purple arrows). Values represent the means ± SD from three independent experiments (*** P ＜0.001).

Journal: Microbiology Spectrum

Article Title: Inhibitory effects of berberine against Streptococcus mutans : an in vitro insight on its anticaries potential

doi: 10.1128/spectrum.00700-25

Figure Lengend Snippet: Effect of BBR on the biofilm formation of S. mutans . In these assays, BBR was incubated with S. mutans in BHIS for 24 h to evaluate its effect on the biofilm formation. ( A ) The crystal violet staining of S. mutans 24 h biofilms under the treatment of BBR. The images were taken from a 24-well cell culture plate. ( B ) The results of the crystal violet staining assay applied to S. mutans biofilms after treated with different concentrations of BBR for 24 h in BHIS. ( C ) SEM micro-images of S. mutans 24 h biofilms on glass coverslips. In the control group, S. mutans bacteria formed dense biofilms covered with large amounts of EPS (red arrows). Disrupted biofilm skeleton (yellow arrows) and enlarged biofilm pores (green arrows) were observed under the treatment of 50 µg/mL of BBR. Only a small number of morphologically irregular S. mutans and cell contents were observed under the treatment of 100 µg/mL and 200 µg/mL of BBR (purple arrows). Values represent the means ± SD from three independent experiments (*** P ＜0.001).

Article Snippet: The bacterial strain S. mutans UA159 was obtained from the American Type Culture Collection (ATCC).

Techniques: Incubation, Staining, Cell Culture, Control, Bacteria

Quantification of S. mutans biofilm after treatment of BBR. ( A ) Double-labeling imaging of S. mutans 24 h biofilms formed on glass coverslips. Live bacteria were green-labeled, and dead bacteria were red-labeled. ( B ) BBR reduced the proportion of live bacteria in S. mutans biofilms. ( C ) The effect of BBR on S. mutans biofilm formation was assessed by measuring the average number of CFU in the biofilm in one well of the culture plate. The analysis was performed using Image J COMSTAT software. Values represent the means ± SD from three independent experiments (*** P ＜0.001).

Journal: Microbiology Spectrum

Article Title: Inhibitory effects of berberine against Streptococcus mutans : an in vitro insight on its anticaries potential

doi: 10.1128/spectrum.00700-25

Figure Lengend Snippet: Quantification of S. mutans biofilm after treatment of BBR. ( A ) Double-labeling imaging of S. mutans 24 h biofilms formed on glass coverslips. Live bacteria were green-labeled, and dead bacteria were red-labeled. ( B ) BBR reduced the proportion of live bacteria in S. mutans biofilms. ( C ) The effect of BBR on S. mutans biofilm formation was assessed by measuring the average number of CFU in the biofilm in one well of the culture plate. The analysis was performed using Image J COMSTAT software. Values represent the means ± SD from three independent experiments (*** P ＜0.001).

Article Snippet: The bacterial strain S. mutans UA159 was obtained from the American Type Culture Collection (ATCC).

Techniques: Labeling, Imaging, Bacteria, Software

Effect of BBR on eradicating the S. mutans mature biofilms. ( A ) Double-labeling imaging of S. mutans mature biofilms treated with BBR. Live and dead bacteria were green-labeled and red-labeled, respectively. ( B ) BBR decreases the proportion of live bacteria in S. mutans mature biofilms. ( C ) The effect of BBR on eradicating the S. mutans mature biofilms was measured by the determination of the average number of CFUs in the biofilm. The S. mutans biofilms were cultured for 24 h without BBR. Then, BBR solution was added to each well for another 24 h. The data were collected at 48 h. The analysis was performed using Image J COMSTAT software. Values represent the means ± SD from three independent experiments (*** P ＜0.001).

Journal: Microbiology Spectrum

Article Title: Inhibitory effects of berberine against Streptococcus mutans : an in vitro insight on its anticaries potential

doi: 10.1128/spectrum.00700-25

Figure Lengend Snippet: Effect of BBR on eradicating the S. mutans mature biofilms. ( A ) Double-labeling imaging of S. mutans mature biofilms treated with BBR. Live and dead bacteria were green-labeled and red-labeled, respectively. ( B ) BBR decreases the proportion of live bacteria in S. mutans mature biofilms. ( C ) The effect of BBR on eradicating the S. mutans mature biofilms was measured by the determination of the average number of CFUs in the biofilm. The S. mutans biofilms were cultured for 24 h without BBR. Then, BBR solution was added to each well for another 24 h. The data were collected at 48 h. The analysis was performed using Image J COMSTAT software. Values represent the means ± SD from three independent experiments (*** P ＜0.001).

Article Snippet: The bacterial strain S. mutans UA159 was obtained from the American Type Culture Collection (ATCC).

Techniques: Labeling, Imaging, Bacteria, Cell Culture, Software

BBR inhibited S. mutans virulence factors. ( A ) Effect of BBR on the biofilm structure of S. mutans observed by CLSM. Double-labeling imaging of S. mutans 24 h biofilm formed on glass coverslips. The fluorescence (SYTO 9) marks the live bacteria, while the red fluorescence (Concanavalin A-TRITC) marks the EPS synthesized by S. mutans . ( B ) Quantification of the amounts of EPS and bacteria in each scanned layer of S. mutans 24 h biofilm without the treatment of BBR. ( C ) Quantification of the amounts of EPS and bacteria in each scanned layer of S. mutans 24 h biofilm with the treatment of 50 µg/mL BBR. ( D ) Quantitative measurement of water-insoluble EPS by anthrone-sulfuric method. ( E ) Measurement of lactic acid production. ( F and G ) Effect of BBR on S. mutans glycolytic pH drop under 1% ( F ) and 0.1% ( G ) glucose. Values represent the means ± SD from three independent experiments (*** P ＜0.001).

Journal: Microbiology Spectrum

Article Title: Inhibitory effects of berberine against Streptococcus mutans : an in vitro insight on its anticaries potential

doi: 10.1128/spectrum.00700-25

Figure Lengend Snippet: BBR inhibited S. mutans virulence factors. ( A ) Effect of BBR on the biofilm structure of S. mutans observed by CLSM. Double-labeling imaging of S. mutans 24 h biofilm formed on glass coverslips. The fluorescence (SYTO 9) marks the live bacteria, while the red fluorescence (Concanavalin A-TRITC) marks the EPS synthesized by S. mutans . ( B ) Quantification of the amounts of EPS and bacteria in each scanned layer of S. mutans 24 h biofilm without the treatment of BBR. ( C ) Quantification of the amounts of EPS and bacteria in each scanned layer of S. mutans 24 h biofilm with the treatment of 50 µg/mL BBR. ( D ) Quantitative measurement of water-insoluble EPS by anthrone-sulfuric method. ( E ) Measurement of lactic acid production. ( F and G ) Effect of BBR on S. mutans glycolytic pH drop under 1% ( F ) and 0.1% ( G ) glucose. Values represent the means ± SD from three independent experiments (*** P ＜0.001).

Article Snippet: The bacterial strain S. mutans UA159 was obtained from the American Type Culture Collection (ATCC).

Techniques: Labeling, Imaging, Fluorescence, Bacteria, Synthesized

The bactericidal mechanism of BBR on S. mutans . ( A ) Effect of BBR on aggregation. ( B ) Membrane depolarization activity of BBR was tested using DiSC3(5). ( C ) TEM micrographs of S. mutans . Values represent the means ± SD from three independent experiments (* P ＜0.05, *** P ＜0.001).

Journal: Microbiology Spectrum

Article Title: Inhibitory effects of berberine against Streptococcus mutans : an in vitro insight on its anticaries potential

doi: 10.1128/spectrum.00700-25

Figure Lengend Snippet: The bactericidal mechanism of BBR on S. mutans . ( A ) Effect of BBR on aggregation. ( B ) Membrane depolarization activity of BBR was tested using DiSC3(5). ( C ) TEM micrographs of S. mutans . Values represent the means ± SD from three independent experiments (* P ＜0.05, *** P ＜0.001).

Article Snippet: The bacterial strain S. mutans UA159 was obtained from the American Type Culture Collection (ATCC).

Techniques: Membrane, Activity Assay

Effects of BBR on S. mutans gene expression. The relative mRNA expressions of gtfB , gtfC , gtfD , ldh , vicR , liaR , and comD were measured by qRT-PCR. S. mutans UA159 16S rRNA was used as an internal control. Values represent the means ± SD from three independent experiments (*** P ＜0.001, ns, not statistically significant compared to the untreated control group).

Journal: Microbiology Spectrum

Article Title: Inhibitory effects of berberine against Streptococcus mutans : an in vitro insight on its anticaries potential

doi: 10.1128/spectrum.00700-25

Figure Lengend Snippet: Effects of BBR on S. mutans gene expression. The relative mRNA expressions of gtfB , gtfC , gtfD , ldh , vicR , liaR , and comD were measured by qRT-PCR. S. mutans UA159 16S rRNA was used as an internal control. Values represent the means ± SD from three independent experiments (*** P ＜0.001, ns, not statistically significant compared to the untreated control group).

Article Snippet: The bacterial strain S. mutans UA159 was obtained from the American Type Culture Collection (ATCC).

Techniques: Gene Expression, Quantitative RT-PCR, Control

Journal: Microbiology Spectrum

Article Title: Inhibitory effects of berberine against Streptococcus mutans : an in vitro insight on its anticaries potential

doi: 10.1128/spectrum.00700-25

Figure Lengend Snippet: Sequences of primers used in this study

Article Snippet: The bacterial strain S. mutans UA159 was obtained from the American Type Culture Collection (ATCC).

Techniques:

Effects of acidic conditions on S. mutans UA159 and SmFtsZ-R68A strains. ( A ) Growth curves of S. mutans UA159 strain and SmFtsZ-R68A strains at different pH levels. Data were collected from three separate and independent experiments. ( B ) Representative CLSM images of biofilms of S. mutans UA159 and SmFtsZ-R68A strains. Live cells (green) were stained with SYTO 9, while dead cells (red) were stained with propidium iodide. Images were captured using a 25× objective lens. Scale bar: 100 µm. ( C ) Survival rate of live cells of biofilm analysis using Leica imaging software. Data represent the means of three independent experiments, analyzed statistically with the Mann−Whitney U test. ‘*’ indicates p < 0.05. ( D ) Overview of the surgical procedure for establishing a dental caries model. ( E–F ) Representative micro-CT and stereomicroscope images of molar regions in the dental caries model are shown in sagittal view. ( G ) Keyes’ scores for the three groups representing levels of dental cavities: Dm refers to moderate dentinal, Ds indicates slight dentinal, and E represents enamel. Data were analyzed using one-way ANOVA in GraphPad Prism 9 (Statistical analysis was performed using one-way ANOVA with GraphPad Prism 9 (GraphPad Software, La Jolla, CA, USA). ‘*’ denotes p < 0.05, while ‘ns’ represents no significant difference.

Journal: bioRxiv

Article Title: Particular Amino Acid on Lateral Interface Maintains FtsZ Function Under Acid Stress in Streptococcus mutans

doi: 10.1101/2025.01.26.634952

Figure Lengend Snippet: Effects of acidic conditions on S. mutans UA159 and SmFtsZ-R68A strains. ( A ) Growth curves of S. mutans UA159 strain and SmFtsZ-R68A strains at different pH levels. Data were collected from three separate and independent experiments. ( B ) Representative CLSM images of biofilms of S. mutans UA159 and SmFtsZ-R68A strains. Live cells (green) were stained with SYTO 9, while dead cells (red) were stained with propidium iodide. Images were captured using a 25× objective lens. Scale bar: 100 µm. ( C ) Survival rate of live cells of biofilm analysis using Leica imaging software. Data represent the means of three independent experiments, analyzed statistically with the Mann−Whitney U test. ‘*’ indicates p < 0.05. ( D ) Overview of the surgical procedure for establishing a dental caries model. ( E–F ) Representative micro-CT and stereomicroscope images of molar regions in the dental caries model are shown in sagittal view. ( G ) Keyes’ scores for the three groups representing levels of dental cavities: Dm refers to moderate dentinal, Ds indicates slight dentinal, and E represents enamel. Data were analyzed using one-way ANOVA in GraphPad Prism 9 (Statistical analysis was performed using one-way ANOVA with GraphPad Prism 9 (GraphPad Software, La Jolla, CA, USA). ‘*’ denotes p < 0.05, while ‘ns’ represents no significant difference.

Article Snippet: The S. mutans UA159 WT strain and its mutants were maintained on BHI media (BBL Becton Dickinson).

Techniques: Staining, Imaging, Software, MANN-WHITNEY, Micro-CT

$Roughness change obtained with a 3D scanning laser microscope (square mean height) of the composite (a) and adhesive (b) disk surfaces, and effective roughness obtained with the laser diffraction pattern (c) after 4-week incubation with S. mutans . *: p < 0.05.$

Journal: Frontiers in Oral Health

Article Title: Effect of a chemically-modified-curcumin on dental resin biodegradation

doi: 10.3389/froh.2024.1506616

Figure Lengend Snippet: Roughness change obtained with a 3D scanning laser microscope (square mean height) of the composite (a) and adhesive (b) disk surfaces, and effective roughness obtained with the laser diffraction pattern (c) after 4-week incubation with S. mutans . *: p < 0.05.

Article Snippet: S. mutans strain 700610 (UA159) was purchased from ATCC® (Manassas, VA, USA).

Techniques: Microscopy, Adhesive, Incubation

Optical photos captured with Laser microscope for composite (a) and adhesive (b) disks before and after 4-week incubation with S. mutans at 50x magnification.

Journal: Frontiers in Oral Health

Article Title: Effect of a chemically-modified-curcumin on dental resin biodegradation

doi: 10.3389/froh.2024.1506616

Figure Lengend Snippet: Optical photos captured with Laser microscope for composite (a) and adhesive (b) disks before and after 4-week incubation with S. mutans at 50x magnification.

Article Snippet: S. mutans strain 700610 (UA159) was purchased from ATCC® (Manassas, VA, USA).

Techniques: Microscopy, Adhesive, Incubation

(a) Percent inhibition of CMC 2.24, CMT-3, curcumin and doxycycline against pure porcine esterase activity at 5th and 10th min after incubation. (b) Percent inhibition of CMC 2.24, CMT-3, curcumin and doxycycline against S. mutans esterase activity at 5th and 10th hour after incubation.

Journal: Frontiers in Oral Health

Article Title: Effect of a chemically-modified-curcumin on dental resin biodegradation

doi: 10.3389/froh.2024.1506616

Figure Lengend Snippet: (a) Percent inhibition of CMC 2.24, CMT-3, curcumin and doxycycline against pure porcine esterase activity at 5th and 10th min after incubation. (b) Percent inhibition of CMC 2.24, CMT-3, curcumin and doxycycline against S. mutans esterase activity at 5th and 10th hour after incubation.

Article Snippet: S. mutans strain 700610 (UA159) was purchased from ATCC® (Manassas, VA, USA).

Techniques: Inhibition, Activity Assay, Incubation

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1) Product Images from "Water-insoluble exopolysaccharide synthesized by glucosyltransferases mediates the antibacterial activity of ClyR against Streptococcus mutans"

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